IDENTIFIERS
Sample ID: |
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TAXONOMY
Phylum | Arthropoda | Subfamily | Scolytinae |
Class | Insecta | Genus | Euwallacea |
Order | Coleoptera | Species | Euwallacea fornicatus |
Family | Curculionidae | Subspecies |
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SPECIMEN DETAILS
Life Stage | Brief Note | ||
Sex | |||
Tissue | |||
Voucher Status |
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COLLECTION DETAILS
Country | Date Collected: | ||
Province/State | Collectors | ||
Region/County | Elevation | ||
Sector | Elv. Accuracy | ||
Exact Site | Brief Note | ||
Latitude | |||
Longitude | |||
Coord Source | |||
Coord Accuracy |
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SEQUENCE: | COI | ||
Sequence ID | GenBank Accession | MT946291 | |
Last Updated | Genome | Mitochondrial genome | |
Locus | |||
Nucleotides | 658 bp | ||
AACTCTATATTTCATTTTTGGTGCATGAGCAGGAATAGTAGGAACATCCCTTAGAGTTTTAATTCGAACCGAATTAGGAA CACCTGGAAAACTAATTATAGATGATCAAATTTTCAATACTATTGTAACAGCCCATGCTTTTATTATAATTTTCTTTATAGT TATACCTATTCTAATTGGTGGATTTGGAAACTGGTTAGTTCCATTAATACTAGGAGCCCCAGATATAGCATTCCCTCGGC TTAATAATATAAGATTTTGGTTATTACCACCAAGATTATCTCTTCTTTTGATAAGAAGAATTATCGACAAAGGAGCAGGA ACAGGATGAACTGTTTATCCTCCTCTAGCAGCAAATATCGCCCATGAAGGAGCTTCAATTGACCTAGCAATTTTTAGACT TCATATATCAGGTGTATCATCAATTCTAGGAGCCATTAATTTTATTTCAACTATTATTAATATACATCCAAAAGGAATAAA ACCTGAACAACTAACCTTATTTACTTGAGCAGTAAAAATCACTGCAATCTTACTATTATTATCTCTACCCGTACTAGCTGG AGGAATCACCATATTACTTACAGATCGAAACATTAACACCTCATTTTTTGATCCAGCAGGAGGTGGAGATCCTGTCCTAT ATCAACATCTATTC | |||
Amino Acids | |||
TLYFIFGAWAGMVGTSLSVLIRTELGTPGKLIMDDQIFNTIVTAHAFIMIFFMVMPILIGGFGNWLVPLMLGAPDMAFP RLNNMSFWLLPPSLSLLLMSSIIDKGAGTGWTVYPPLAANIAHEGASIDLAIFSLHMSGVSSILGAINFISTIINMHPKG MKPEQLTLFTWAVKITAILLLLSLPVLAGGITMLLTDRNINTSFFDPAGGGDPVLYQHLF | |||
Illustrative Barcode | |||
 | |||
Length | Pcr Primers | Read(5’-3’) | Status | References |
658 bp | LCO1490/ HCO2198 | Forward primer： GGTCAACAAATCATAAAGATATTGG Reverse primer： TAAACTTCAGGGTGACCAAAAAATCA | high qual | 【1】【2】 |
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SEQUENCE: | 16S | ||
Sequence ID | GenBank Accession | MT946906 | |
Last Updated | Genome | ||
Locus | |||
Nucleotides | 305 bp | ||
AAACTACTGTCTCAATTAAAATTTTTTAGAATTTTATTATTTTGTAAAAAGGCTAAAATTATTATAAAAGACGAGAAGACCCTATAGA GTTTTATAATTTGTATAGAAAGGGTAATTAGAATAGATTTCTTTATTTGTTTAAATTATTTGGTTGGGGTGATTGAAAAACTTAATTAA CTTTTTTTCAAAGATTACATATATTTATGAATATTTGATCCTTATAAAAGATTAAAAGAAAAAATTACCTTAGGGATAACAGCGTTATT TTTCTTGAGAGTTCTTATCGAAAGAAGAGTTTGCGACCT | |||
Amino Acids | |||
Illustrative Barcode | |||
 | |||
Length | Pcr Primers | Read(5’-3’) | Status | References |
305 bp | 16S-F/ 16S-R | Forward primer： TRACTGTRCAAAGGTAGC Reverse primer： TTAATTCAACATCGAGGTC | high qual | 【2】【3】 |
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SEQUENCE: | 28S ribosomal RNA gene | ||
Sequence ID | GenBank Accession | MT940909 | |
Last Updated | Genome | ||
Locus | |||
Nucleotides | 1037 bp | ||
AAGGGCACCACAAGAACGCGTGGAGCATGTGGCTTAATTTGACTCAACGCGGGAAATCTTACCGGGTCCGGACACACTGAGGATTG ACAGATATACGACCAGTGTTCGCTTCGGCGTCTCTGCTCTGAAAGATGCTAGTTCTTTCCTGATTATGTGATAGGTGGTGCATGGCCG TTCTTAGTTCGTGGAATGATCTGTCTGCTTAATTGCGTATCAATATACCAAACCTAATATACCCGTAAAACCACCAGGTTCGTGACCCC CCTTCGGGGCGTGTGTCGCACTCCTGCTATACCCACAGGTCTCAGATAGCAACCAACGTGACCGTACTCTATTGTTGCAGTGAAATA GTGGGTGGTTTCGGCGGCCCCCACCCCCTGCTTATCGTATCTTCGCTTCGGCGGAGTTATACACATTAAACTATAGAGACCGCTGTTT CTTCTTTAAACCAGAGGAAGGATACGGCAATAACAGGTCTGTGATGCCCTCAGATGTTCCGGGCTGCACACGTGCTACAATGATCAT TGCACTGTGCATCTAACCCAATGCGCGCGGACGCCACGCTTTGTTCGGTTCGCCGAATGGAATGTTGATTGCGACCGCGCCGAACCT ACTTCGAAAGTAACATTTCTCAGTGGGTAATCCATTAGAAGTAATGACTCGCATAGACCATGGCACACAAAATGTACGCGCAGGTCT ACCCGGCTCGCCTTTGTGTGAGTGCAGTGCGTAGCTTGTTGTTTCGTACGTGCCACCTCGTATTAATTCGTACGTGGGGACAGATCAT TGTTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTTGTACGGGTCTTTGGTTCAACATACCACCCGGAATACGTCCCTGCCCTTTG TACACACCGCCCGTCGCTCTTACCGATGGATTATACTATGAATCTATAGGACTGCCAAAGCTGCGGCTCGCCGCGCTTAGTGGAAAT ATATATGAATAGTGTGATCTAAAGGAAAGAGAAGTCGTAACAAGGCATCGGTAGGTGAACCTGCAGAAGGATCA | |||
Amino Acids | |||
Illustrative Barcode | |||
 | |||
Length | Pcr Primers | Read(5’-3’) | Status | References |
1037 bp | 28S Rd 4.8a / 28S Rd 7b1 | Forward primer： ACCTATTCTCAAACTTTAAATGG Reverse primer： GACTTCCCTTACCTACAT | high qual | 【2】【4】 |
REFERENCES
[1] Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol. 1994 Oct;3(5):294-9. PMID: 7881515. [2] 黄卫东,梁建锋,彭锋等.基于通用引物的多重PCR对小圆胸小蠹的分子鉴定[J].环境昆虫学报,2022,44(04):994-1001. [3] Schulmeister, S. (2003). Simultaneous analysis of basal Hymenoptera (Insecta): introducing robust-choice sensitivity analysis. Biological Journal of the Linnean Society, 79(2), 245–275. doi:10.1046/j.1095-8312.2003.00233.x [4] Robertson, J. A., Ślipiński, A., Hiatt, K., Miller, K. B., Whiting, M. F., & Mchugh, J. V. (2012). Molecules, morphology and minute hooded beetles: a phylogenetic study with implications for the evolution and classification of Corylophidae (Coleoptera: Cucujoidea). Systematic Entomology, 38(1), 209–232. doi:10.1111/j.1365-3113.2012.00655.x |
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