IDENTIFIERS
Sample ID: |
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TAXONOMY
Phylum | Arthropoda | Subfamily | |
Class | Insecta | Genus | Cydia |
Order | Lepidoptera | Species | Cydia pomonella |
Family | Tortricidae | Subspecies |
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SPECIMEN DETAILS
Life Stage | Brief Note | ||
Sex | |||
Tissue | |||
Voucher Status |
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COLLECTION DETAILS
Country | Date Collected: | ||
Province/State | Collectors | ||
Region/County | Elevation | ||
Sector | Elv. Accuracy | ||
Exact Site | Brief Note | ||
Latitude | |||
Longitude | |||
Coord Source | |||
Coord Accuracy |
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SEQUENCE: | COI | ||
Sequence ID | GenBank Accession | KJ789183 | |
Last Updated | Genome | Mitochondrial genome | |
Locus | |||
Nucleotides | 435 bp | ||
CCCCCATCTATTATACTTTTAATTTCAAGCAGAATCGTTGAAAATGGAGCAGGAACAGGATGAACAGTGTACCCCCCAC TTTCTTCTAATATTGCCCATAGAGGAAGATCTGTTGATTTAGCTATTTTTTCTTTACATTTAGCGGGTATTTCTTCTATTTTA GGAGCTGTTAATTTTATTACAACTATTATTAATATACGACCTAATAATATATCATTAGATCAAATACCATTATTTGTTTGAG CTGTAGGAATTACAGCTCTTTTACTTCTTTTATCATTACCAGTATTAGCAGGTGCTATTACTATGCTTCTTACAGATCGAAA TCTTAATACATCATTTTTTGACCCTGCTGGTGGAGGTGATCCTATTCTCTACCAACACTTATTTTGATTTTTCGGTCATCCTG AAGTTTATATTTTAATTTTACCGGGA | |||
Amino Acids | |||
PPSIMLLISSSIVENGAGTGWTVYPPLSSNIAHSGSSVDLAIFSLHLAGISSILGAVNFITTIINMRPNNMSLDQMPLFVW AVGITALLLLLSLPVLAGAITMLLTDRNLNTSFFDPAGGGDPILYQHLFWFFGHPEVYILILPG | |||
Illustrative Barcode | |||
 | |||
Length | Pcr Primers | Read(5’-3’) | Status | Reference |
435 bp | mtD7.2F/ mtD9.2R | Forward primer：GGAGGATTTGGAAATTGATTAGTTCC Reverse primer：CCCGGTAAAATTAAAATATAAACTTC | high qual | 【1】【3】 |
C1-J-1751/ C1-N-2191 | Forward primer：GGATCACCTGATATAGCATTCCC Reverse primer：CCCGGTAAAATTAAAATATAAACTTC | high qual | 【2】【3】 |
SEQUENCE: | COII | ||
Sequence ID | GenBank Accession | KJ789279 | |
Last Updated | Genome | Mitochondrial genome | |
Locus | |||
Nucleotides | 682 bp | ||
ATGGCAACTTGATCTAATCTTAATCTTCAAAATAGAGCTTCCCCATTAATAGAACAAATTATTTTTTTTCATGATCA TACTTTAATTATTTTAATCATAATTACAATTTTAGTTGGATATTTAATAATTAGTTTATTTTTTAATTCTCACATTAATC GATTTTTATTAGAAGGTCAAATAATTGAATTAATTTGAACAATTTTACCAGCAATTACTTTAATTTTTATTGCTTTACC ATCATTACGTTTATTATACTTATTAGATGAATTAAATAACCCCCTAATTACATTAAAATCTATTGGTCATCAATGATA TTGAAGTTATGAATATTCAGATTTTCACAATATTCAATTTGATTCATATATAATCCCCCAAAATGAAATAAATAATA ATAGTTTTCGTCTCTTGGATGTAGATAATCGAATTATTATTCCTATAAATAATCAAATTCGAATCATAGTTACAGCA ACAGATGTCATCCATTCCTGAACAATTCCTTCATTAGGGGTAAAAGTAGATGCTAATCCGGGTCGACTCAATCAAA CTAATTTTTTTATTAATCGTCCTGGAATTTTTTATGGACAATGTTCCGAAATTTGTGGAGCAAATCATAGCTTTATACC TATTGTAATTGAAAGTGTTTCAATTAAAAATTTTATTAATTGAATTAATAATTATTCTT | |||
Amino Acids | |||
MATWSNLNLQNSASPLMEQIIFFHDHTLIILIMITILVGYLMISLFFNSHINRFLLEGQMIELIWTILPAITLIFIALP SLRLLYLLDELNNPLITLKSIGHQWYWSYEYSDFHNIQFDSYMIPQNEMNNNSFRLLDVDNRIIIPMNNQIRIM VTATDVIHSWTIPSLGVKVDANPGRLNQTNFFINRPGIFYGQCSEICGANHSFMPIVIESVSIKNFINWINNYS | |||
Illustrative Barcode | |||
 | |||
Length | Pcr Primers | Read(5’-3’) | Status | Reference |
682 bp | TL2-J-3037/ TK-N-3785 | Forward primer： ATGGCAGATTATATGTAATGG Reverse primer： GTTTAAGAGACCAGTACTTG | high qual | 【2】【3】 |
SEQUENCE: | Cytb | ||
Sequence ID | GenBank Accession | KJ789225 | |
Last Updated | Genome | Mitochondrial genome | |
Locus | |||
Nucleotides | 710 bp | ||
AGGAAATATATTAGTAAATTGAATTTGAGGAGGATTTGCCGTTGATAATGCAACATTAACACGATTTTATACTTTCCATTTTCTTCTT CCATTTATTCTTGCAATAATAACAATAATTCATTTATTATTTTTACATCAAACAGGCTCAAATAATCCATTAGGTATTAATAGAAATTT AGATAAAATTCCTTTTCATCCTTTTTTTTCTTTTAAGGATTTAGTCGGATTTTTAATTATACTATTTATTTTAATTATATTAACTATAATT AATCCATATCTTTTAGGAGACCCTGATAATTTTATTCCAGCAAATCCTTTAGTTACTCCTGTTCATATTCAACCTGAATGATATTTCTT ATTTGCTTATGCAATTTTACGATCTATCCCCAATAAATTAGGAGGAGTAATTGCTTTAGTTATATCCATTTTAATTTTAATTTTTTTACC TATAACTTTTAATAAAAAAATTCAAGGAATTCAATTTTACCCTATTAACCAAATTTTATTTTGATCTATAGTAACTACCGTTATTCTACT AACTTGAATTGGAGCCCGACCTGTAGAAGAACCTTATATTATTACAGGACAACTTCTCACTGTATTATATTTTTCTTATTTCATTTTTA ATCCTCTATTAAATAAATACTGAGACAACTTAATTTTTAATTAATTAATTAATGAGCTTGTTATAAGCATTTGTTTTGAAAACT | |||
Amino Acids | |||
GNMLVNWIWGGFAVDNATLTRFYTFHFLLPFILAMMTMIHLLFLHQTGSNNPLGINSNLDKIPFHPFFSFKDLVGFLIMLFILIMLT MINPYLLGDPDNFIPANPLVTPVHIQPEWYFLFAYAILRSIPNKLGGVIALVMSILILIFLPMTFNKKIQGIQFYPINQILFWSMVTTVI LLTWIGARPVEEPYIITGQLLTVLYFSYFIFNPLLNKYWDNLIFN | |||
Illustrative Barcode | |||
 | |||
Length | Pcr Primers | Read(5’-3’) | Status | Reference |
710 bp | F/ R | Forward primer： TATGTTTTACCATGAGGTCAAATATC Reverse primer： TATTTCTTTCTTAAGTTTTCAAAAC | high qual | 【2】【3】 |
REFERENCE
[1] 董昆. 基于COI基因的苹果蠹蛾地理种群遗传差异分析及幼虫快速分子鉴定[D].西北农林科技大学,2010. [2] 李玉婷. 基于线粒体基因标记的我国苹果蠹蛾遗传多样性与入侵来源分析[D].西北农林科技大学,2013. [3] Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H., & Flook, P. (1994). Evolution, Weighting, and Phylogenetic Utility of Mitochondrial Gene Sequences and a Compilation of Conserved Polymerase Chain Reaction Primers. Annals of the Entomological Society of America, 87(6), 651–701. doi:10.1093/aesa/87.6.651 |
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