IDENTIFIERS
Sample ID: |
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TAXONOMY
Phylum | Arthropoda | Subfamily | |
Class | Insecta | Genus | Phenacoccus |
Order | Hemiptera | Species | Phenacoccus solenopsis |
Family | Pseudococcidae | Subspecies |
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SPECIMEN DETAILS
Life Stage | Brief Note | ||
Sex | |||
Tissue | |||
Voucher Status |
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COLLECTION DETAILS
Country | Date Collected: | ||
Province/State | Collectors | ||
Region/County | Elevation | ||
Sector | Elv. Accuracy | ||
Exact Site | Brief Note | ||
Latitude | |||
Longitude | |||
Coord Source | |||
Coord Accuracy |
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SEQUENCE: | 28S ribosomal RNA gene | ||
Sequence ID | GenBank Accession | MK873095 | |
Last Updated | Genome | ||
Locus | |||
Nucleotides | 708 bp | ||
GAGCCCGTGAATCCGGGCGGCAGAATTCAGAGACGATGGCGGCAACGTTGTCGGTTCGATATTTCTGTCGCCGGCACG GACGTCGCGACCCGTTTGGTGTCGGTCTCAGGAGACGCGTTTCACGTTCGTCGACGCGTCGTCTGCCTCGGTAGGCGCG CGTTGCGAGTACGCGTTCGTGTTCCGGCCGACTCGCCAGACGGTAGGTTAATGGTGGCCGCGGCGGTCGTTCGCTTCGC GGCGATCAGCGCCGTCCGCGGTGCCGGTTTGCGACGAATCTTCGGGCCTCTTTCCGACCCGTCTTGAAACACGGACCAA GGAGTCTAACGTGCGCGCGAGTCGCGGGGTGTCGAGCGGCGGTCGTGTTTCGGCACGACCGTCGTTCGTTACGACGCG AAACCCGTACCGTTCATTCGGAAGGCGCAATGAAAGTGAAATGGGGAAGATGGCGTTACCGAGTTCACGTTTCGCGAC TCGATCGCGACCGGTCGCCCGCATTCCCAGGGCGTCCCAACGGCTCGGCGGCGTTGCGGATCGTCGTTCCTTCGGGAAC GGCGGTCGGGCGTCGTCGGTCCGGGGCGCACCTACAGCGTGCACGTTGGTACCCGAAAGATGGTGAACTATGCCCGGC CAGGATGAAGTCAGGGGAAACCCTGATGGAGGTCCGCAGCGATTCTGACGTGCAAATCGATCGTCTGAGCTGGGTATA GG | |||
Illustrative Barcode | |||
Length | Pcr Primers | Read(5’-3’) | Status | References |
708 bp | S3660/ A335 | Forward primer: GAGAGTTMAASAGTACGTGAAAC Reverse primer: TCGGARGGAACCAGCTACTA | high qual | 【1】 |
28S-rDNA-F/ 28S-rDNA-R | Forward primer: GTAGCCAAATGCCTCGTCA Reverse primer: CACAATGATAGGAAGAGCC | high qual | 【5】 |
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SEQUENCE: | COI | ||
Sequence ID | GenBank Accession | GQ903580 | |
Last Updated | Genome | Mitochondrial genome | |
Locus | |||
Nucleotides | 712 bp | ||
ATATCACAAATTATAAATCAAGAAACAGGAAAAATTGAAATTTTTAGAAAAATTAATATAATTTATGCTATAATTTCAATTGGAATTTTAGG ATTTATTGTTTGAGCTCATCATATATTTACTATTGGTTTAGATATTGATACTCAATTATATTTTATATCAGCAACTATAATTATTGCAATTCCTA CAAGAATTAAAATTTTTAGATGATTAATAACACTTAATGGAAAAAAAATATTTAATTCATCAACTAATTTTTGATCTATTGGATTTATTATTAT ATTTACTTTAGGGGGATTAACTGGTATTATTCTTTCAAACTCTATTATTGATATTAACTTACATGATACATATTTCGTTGTAGCTCATTTTCATT ATGTTTTATCTATGGGAGTAATTTTTTCTATTTTTTCAAGAATTATTTTTTGATTTCCTTTATTAACTAATTTAATATTAAATAATAATTGATTAA AAATTAATTTTTTTAATTTATTTTTATCAATTAATTTAACTTTTTTCCCTCAACATTTTTTAGGACTAAGAGGTATACCTCGACGATATATTATA TATTCAGATTATTTTATTATATGAAACAATATTTCATCTTTAGGATCTTTAATAACAATTTTATTTACATTTATTTTTATTTATATTATTATTGAAA GAATATTAGCTAAACGAACAATAATTTTTAAATTTAAATTTTTCAATA | |||
Amino Acids | |||
MSQIMNQETGKIEIFSKINMIYAMISIGILGFIVWAHHMFTIGLDIDTQLYFMSATMIIAIPTSIKIFSWLMTLNGKKMFNSSTNFWSIGFIIMFTLGG LTGIILSNSIIDINLHDTYFVVAHFHYVLSMGVIFSIFSSIIFWFPLLTNLMLNNNWLKINFFNLFLSINLTFFPQHFLGLSGMPRRYIMYSDYFIMWNN ISSLGSLMTILFTFIFIYIIIESMLAKRTMIFKFKFFN | |||
Illustrative Barcode | |||
Length | Pcr Primers | Read(5’-3’) | Status | References |
712 bp | C1-J-2195/ L2-N-3014 | Forward primer: TTGATTTTTTGGTCAT CCAGAAGT Reverse primer: TCCAATGCACT AATCTGCCATATTA | high qual | 【2】【3】 |
630 bp | PZTCOIF1/ PZTCOIR1 | Forward primer: TTTTTGGATTTTGATCAGG Reverse primer: CTTCTGGATGTCCAAAGAATC | high qual | 【4】 |
546 bp | PSZTF1/ PSZTR1 | Forward primer: TTTTTGGATTTTGATCAGG Reverse primer: TAGCTCTTGAAAGTACTGGAATTGAAAC | high qual | 【4】 |
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SEQUENCE: | COI | ||
Sequence ID | GenBank Accession | KJ187574 | |
Last Updated | Genome | Mitochondrial genome | |
Locus | |||
Nucleotides | 764 bp | ||
ATTATATCACAAATTATAAATCAAGAAACAGGAAAAATTGAAATTTTTAGAAAAATTAATATAATTTATGCTATAATTTCAATTGGAATTTTA GGATTTATTGTTTGAGCTCATCATATATTTACTATTGGTTTAGATATTGATACTCAATTATATTTTATATCAGCAACTATAATTATTGCAATTCC TACAAGAATTAAAATTTTTAGATGATTAATAACACTTAATGGAAAAAAAATATTTAATTCATCAACTAATTTTTGATCTATTGGATTTATTATTA TATTTACTTTAGGGGGATTAACTGGTATTATTCTTTCAAACTCTATTATTGATATTAACTTACATGATACATATTTCGTTGTAGCTCATTTTCATT ATGTTTTATCTATGGGAGTAATTTTTTCTATTTTTTCAAGAATTATTTTTTGATTTCCTTTATTAACTAATTTAATATTAAATAATAATTGATTAAA AATTAATTTTTTTAATTTATTTTTATCAATTAATTTAACTTTTTTCCCTCAACATTTTTTAGGACTAAGAGGTATACCTCGACGATATATTATATA TTCAGATTATTTTATTATATGAAACAATATCTCATCTTTAGGATCTTTAATAACAATTTTATTTACATTTATTTTTATTTATATTATTATTGAAAGA ATATTAGCTAAACGAACAATAATTTTTAAATTTAAATTTTTCAATATTGAATGATTAATAAATTCTCCAAATTTAAATCATTCATTTAATGAAAA | |||
Amino Acids | |||
IMSQIMNQETGKIEIFSKINMIYAMISIGILGFIVWAHHMFTIGLDIDTQLYFMSATMIIAIPTSIKIFSWLMTLNGKKMFNSSTNFWSIGFIIM FTLGGLTGIILSNSIIDINLHDTYFVVAHFHYVLSMGVIFSIFSSIIFWFPLLTNLMLNNNWLKINFFNLFLSINLTFFPQHFLGLSGMPRRYIMYS DYFIMWNNISSLGSLMTILFTFIFIYIIIESMLAKRTMIFKFKFFNIEWLMNSPNLNHSFNE | |||
Illustrative Barcode | |||
Length | Pcr Primers | Read(5’-3’) | Status | References |
764 bp | PCO-F1/ LEP-R1 | Forward primer: CCTTCAACTAATCATAAAAATATYAG Reverse primer: TAAACTTCTGGATGTCCAAAAAATCA | high qual | 【6】【7】 |
REFERENCES
[1] 林凌鸿,郑丽祯,史梦竹等.福建新记录入侵害虫木瓜秀粉蚧的分子检测鉴定[J].果树学报,2019,36(09):1130-1139.DOI:10.13925/j.cnki.gsxb.20180526. [2] Frohlich, D. R., Torres-Jerez, I., Bedford, I. D., Markham, P. G., & Brown, J. K. (1999). A phylogeographical analysis of the Bemisia tabaci species complex based on mitochondrial DNA markers. Molecular Ecology, 8(10), 1683–1691. doi:10.1046/j.1365-294x.1999.00754.x [3] 陈哲,张姜,傅杭飞等.基于形态特征和线粒体COI基因探讨扶桑绵粉蚧物种的有效性并记述一体色变异型扶桑绵粉蚧[J].生物多样性,2012,20(04):443-450. [4] 田虎,李小凤,万方浩等.利用种特异性COI引物(SS-COI)鉴别扶桑绵粉蚧[J].昆虫学报,2013,56(06):689-696.DOI:10.16380/j.kcxb.2013.06.010. [5] 赵静,孙洋,谭永安等.基于COI及28SrDNA序列分析的扶桑绵粉蚧地理种群的遗传分化研究[J].棉花学报,2014,26(02):130-137. [6] Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H., & Flook, P. (1994). Evolution, Weighting, and Phylogenetic Utility of Mitochondrial Gene Sequences and a Compilation of Conserved Polymerase Chain Reaction Primers. Annals of the Entomological Society of America, 87(6), 651–701. doi:10.1093/aesa/87.6.651 [7] 吴福中,王徐玫,李惠萍等.扶桑绵粉蚧及其近似种的DNA条形码鉴定[J].植物检疫,2020,34(02):42-47.DOI:10.19662/j.cnki.issn1005-2755.2020.02.009. |
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